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Negative control; omission of the primary antibody or only the primary antibody applied. No immunoreactivity is detected within the smooth muscle cell layer (arrow points). Only auto-fluorescence in lamina elastica interna (arrow) is detected. LEI; lamina elastica interna. Vascular wall structures; example of immunohistochemical staining on human artery showing the different wall structures. ADV; adventitial layer, LEI; lamina elastica interna, SML; smooth muscle cell layer.
