Table 3.
Antibody array results
| Protein name (phosphorylated residue) | Fold change antibody array | P -value | Orthology | Fold change peptide array |
|---|---|---|---|---|
| 4E-BP1 (Phospho-Ser65) |
−1.43712 |
0.00137 |
93% |
N/A |
| 4E-BP1 (Phospho-Thr70) |
−1.36207 |
0.013516 |
86% |
N/A |
| AKT (Phospho-Ser473) |
1.579649 |
0.002667 |
100% |
1.61614 |
| AKT (Phospho-Thr308) |
1.898775 |
0.003797 |
100% |
1.31019 |
| AKT1 (Phospho-Ser124) |
−1.47999 |
0.000402 |
100% |
N/A |
| AKT1 (Phospho-Thr450) |
−1.5584 |
0.000476 |
100% |
N/A |
| AKT1S1 (Phospho-Thr246) |
−1.79817 |
2.22E-05 |
0% |
N/A |
| AMPK1 (Phospho-Thr174) |
−1.22623 |
0.0154 |
100% |
−1.09134 |
| AMPKbeta1 (Phospho-Ser182) |
−1.34017 |
0.029026 |
93% |
−1.10925 |
| BAD (Phospho-Ser112) |
1.40376 |
0.012781 |
78% |
N/A |
| BAD (Phospho-Ser134) |
1.380989 |
0.020394 |
0% |
N/A |
| BAD (Phospho-Ser136) |
1.29749 |
0.039652 |
0% |
N/A |
| eIF4E (Phospho-Ser209) |
−1.33384 |
0.030631 |
100% |
N/A |
| ERK3 (Phospho-Ser189) |
1.210088 |
0.019255 |
100% |
4.01876 |
| GSK3 beta (Phospho-Ser9) |
2.974451 |
0.002007 |
100% |
1.12689 |
| IR (Phospho-Tyr1355) |
3.187483 |
2.62E-05 |
53% |
N/A |
| IR (Phospho-Tyr1361) |
1.685564 |
0.012336 |
93% |
N/A |
| P70S6K (Phospho-Ser371) |
−1.73418 |
0.000111 |
100% |
N/A |
| P70S6K (Phospho-Ser418) |
−1.58607 |
0.000109 |
100% |
N/A |
| P70S6K (Phospho-Ser424) |
−1.39268 |
0.037721 |
100% |
N/A |
| P70S6K (Phospho-Thr229) |
−1.44379 |
0.000416 |
100% |
N/A |
| P70S6K (Phospho-Thr389) |
−1.84347 |
0.009943 |
100% |
−1.12207 |
| P70S6K (Phospho-Thr421) |
−1.22221 |
0.013127 |
100% |
N/A |
| P70S6k-beta (Phospho-Ser423) |
1.60054 |
0.000107 |
100% |
N/A |
| P90RSK (Phospho-Ser380) |
1.255345 |
0.04783 |
100% |
−1.12470 |
| PDK1 (Phospho-Ser241) |
1.632697 |
0.002201 |
93% |
−1.01557 |
| PI3-kinase p85-alpha (Phospho-Tyr607) |
−1.3092 |
0.038374 |
87% |
−1.34043 |
| PIP5K (Phospho-Ser307) |
−1.2407 |
0.026195 |
100% |
N/A |
| PTEN (Phospho-Ser370) |
1.491624 |
0.002252 |
100% |
N/A |
| PTEN (Phospho-Ser380) | 1.533421 | 0.005982 | 100% | 1.12240 |
Statistically significant (p < 0.05) phosphospecific antibody array analysis results of Salmonella Typhimurium infected muscle samples. One week post-infection samples were compared to 24 h post-infection samples to find changes in infected muscle over time. Antibodies due to being bound to phosphorylated protein had a statistically significant difference in fluorescent signal are shown. Fold Change Antibody Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the antibody array. Orthology indicates the % similarity between human and chicken at the 15 amino acid region flanking the phosphorylation residue. Fold Change Peptide Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the peptide array. N/A indicates the exact phosphorylation target residue on the antibody array was not present on the peptide array.