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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Eur J Immunol. 2012 Jun;42(6):1639–1647. doi: 10.1002/eji.201142066

Figure 5.

Figure 5

Sin1 is not required for CD4+ TH effector cell differentiation.

a) Purified CD4+ T cells from chimeric mice were stimulated with anti-CD3 plus anti-CD28 under indicated TH1 TH17, andTH2 polarizing conditions as indicated and then analyzed by flow cytometry. Plots are gated on donor T cells from WT or Sin1−/− (KO) chimeric mice. Plots shown are representative of two independent experiments.

b) Sin1+/+ (WT) or Sin1−/− (KO) CD4+ T cells were stimulated with anti-CD3 for 3 minutes and Akt S473 phosphorylation was measured by flow cytometry. Histograms shown are pre-gated on CD45.2+ donor cells and are representative of two independent experiments.

c) Sin1−/− (KO) or Sin1+/+ (WT) CD4+ T cells were stimulated with anti-CD3 for 0, 15 or 30 minutes and Akt T450 phosphorylation and total Akt was measured by immunoblotting. Total ERK2 serves as a loading control.