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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Eur J Immunol. 2012 Jun;42(6):1639–1647. doi: 10.1002/eji.201142066

Figure 6.

Figure 6

Sin1 and mTOR differentially regulate TGF-β dependent Treg differentiation.

a) Sin1+/+ (WT) or Sin1−/− (KO) CD4+ T cells were stimulated in vitro with anti-CD3 plus anti-CD28 antibodies in the presence or absence of 1 ng/ml TGF-β. After four days Foxp3 expressing cells were enumerated by flow cytometry. Data shown are representative of two independent experiments.

b) Sin1+/+ CD4+ T cells were stimulated in vitro with anti-CD3 plus anti-CD28 antibodies plus 1 ng/ml TGF-β in the presence of rapamycin (Rapa. 30 nM), pp242 (100 nM) or carrier (ctrl.). The proportion of Foxp3+ cells generated after four days was measured by flow cytometry. Data are representative of two independent experiments.

c) Sin1+/+ CD4+ T cells were activated with anti-CD3 in the presence of indicated inhibitors and assayed for induction of S6 p-S235/S236 and Akt p-S473 by flow cytometry. Data are representative of two independent experiments.