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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Biomol Screen. 2013 Jan 30;18(5):588–598. doi: 10.1177/1087057112474690

Figure 1.

Figure 1

Reporter fusions used in high-throughput screen and structure of OSSL_051168 compared with compound identified in the screen. (A) Top: Primary screen, RhaS-activated rhaB-lacZ fusion, shown in uninduced, (−)rhamnose state (left) and induced (+)rhamnose state (right). Bottom: Secondary screen, LacI-repressed hts-lacZ control fusion, shown in uninduced, (−)IPTG state (left) and induced (+)IPTG state (right). Gray rectangles: RhaS and LacI (lacO) binding sites. Gray arrows: lacZ gene expressed from rhaBAD or synthetic hts promoter. Thick black/gray line: Promoter region DNA. Right angle black arrow: transcription start sites. Rounded gray shapes: RNAP, RhaS or LacI proteins. Wavy lines: active transcription in the presence of inducers L-rhamnose (Rha) and IPTG. (B) The hit from the high throughput screen, 1-ethyl-4-nitromethyl-3-quinolin-2-yl-4H-quinoline. (C) OSSL_051168, 1-butyl-4-nitromethyl-3-quinolin-2-yl-4H-quinoline.