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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Biomol Screen. 2013 Jan 30;18(5):588–598. doi: 10.1177/1087057112474690

Figure 3.

Figure 3

Effect of OSSL_051168 on DNA binding by RhaS-GB1201b and GB1b-RhaR. The DNA bound (assayed by EMSA) at the lowest concentration of inhibitor (OSSL_051168) was set to 100%, and binding in all other cases is represented relative to that value. Inhibitor concentrations ranged from 10 to 650 µM, with serial two-fold dilutions. Results are the average of three independent experiments. (A) RhaS-GB1201b. Final concentrations of DNA and protein were 2.5 nM and 2.4 µM, respectively. (B) GB1b-RhaR. Final concentrations of DNA and protein were 1.3 nM and 2.2 µM, respectively.