Table 2.
Assay performance data for the quantitation of QCLL, QCL, QCM and QCH belmostat and metabolite concentrations in human plasma.
| Analyte | Concentration (ng/mL) | Accuracy (%) | Intra-assay precision (%) | Inter-assay precision (%) |
|---|---|---|---|---|
| Belinostat | 30 (QCLL) | 96.8 | 5.9 | 0.9 |
| 100 (QCL) | 99.4 | 6.3 | * | |
| 2500 (QCM) | 101.0 | 4.2 | * | |
| 4000 (QCH) | 96.2 | 3.4 | * | |
|
| ||||
| Belinostat-glucuronide | 30 (QCLL) | 94.8 | 7.6 | 12.6 |
| 100 (QCL) | 101.2 | 10.2 | 3.1 | |
| 2500 (QCM) | 104.4 | 8.5 | 2.4 | |
| 4000 (QCH) | 95.3 | 7.79 | 2.9 | |
|
| ||||
| Methyl-belinostat | 30 (QCLL) | 96.7 | 9.3 | 5.4 |
| 100 (QCL) | 103.2 | 8.0 | 2.7 | |
| 2500 (QCM) | 101.3 | 8.4 | 2.5 | |
| 4000 (QCH) | 92.0 | 7.4 | 7.4 | |
|
| ||||
| M21 | 30 (QCLL) | 93.6 | 9.3 | 2.8 |
| 100 (QCL) | 97.9 | 13.7 | * | |
| 2500 (QCM) | 96.4 | 8.3 | 2.3 | |
| 4000 (QCH) | 97.7 | 9.7 | 3.0 | |
|
| ||||
| M24 | 30 (QCLL) | 99.7 | 13.6 | 6.4 |
| 100 (QCL) | 93.4 | 12.7 | 3.9 | |
| 2500 (QCM) | 95.9 | 7.8 | * | |
| 4000 (QCH) | 96.0 | 10.8 | * | |
|
| ||||
| M26 | 30 (QCLL) | 102.7 | 11.1 | 1.0 |
| 100 (QCL) | 102.5 | 9.4 | 5.0 | |
| 2500 (QCM) | 98.0 | 8.3 | 4.4 | |
| 4000 (QCH) | 97.3 | 10.3 | * | |
N=18; 6-fold results, each in 3 separate runs, for each concentration.
*
The mean square of the within runs was greater than the mean square of the between runs, indicating that there was no significant additional variation due to the performance of the assay in different runs[6].