Table 5.
Assay performance data of QCLL, QCL, QCM and QCH belinostat and metabolite concentrations in citrate plasma quantitated against a heparinized plasma calibration curve.
| Analyte | Concentration (ng/mL) | Accuracy (%) | Precision (%) |
|---|---|---|---|
| Belinostat | 30 (QCLL) | −11.4 | 11.3 |
| 100 (QCL) | −9.2 | 5.2 | |
| 2500 (QCM) | 10.5 | 2.7 | |
| 4000 (QCH) | 12.5 | 1.4 | |
|
| |||
| Belinostat-glucuronide | 30 (QCLL) | −17.7 | 6.2 |
| 100 (QCL) | −9.9 | 6.6 | |
| 2500 (QCM) | −7.3 | 5.4 | |
| 4000 (QCH) | −11.5 | 3.4 | |
|
| |||
| Methyl-belinostat | 30 (QCLL) | −14.7 | 9.7 |
| 100 (QCL) | −9.3 | 5.1 | |
| 2500 (QCM) | −4.9 | 6.5 | |
| 4000 (QCH) | −9.7 | 3.9 | |
|
| |||
| M21 | 30 (QCLL) | −1.7 | 9.1 |
| 100 (QCL) | −1.4 | 11.1 | |
| 2500 (QCM) | 2.7 | 5.4 | |
| 4000 (QCH) | 4.1 | 6.5 | |
|
| |||
| M24 | 30 (QCLL) | −2.3 | 13.7 |
| 100 (QCL) | −5.5 | 5.7 | |
| 2500 (QCM) | 10.1 | 6.5 | |
| 4000 (QCH) | 5.8 | 6.8 | |
|
| |||
| M26 | 30 (QCLL) | 8.7 | 6.9 |
| 100 (QCL) | 8.2 | 3.9 | |
| 2500 (QCM) | 10.0 | 2.4 | |
| 4000 (QCH) | 8.1 | 4.7 | |
N=6.