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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Bone Miner Res. 2013 Jun;28(6):1478–1488. doi: 10.1002/jbmr.1882

Figure 3. 1,25D effects on miR host and target gene expression under different HOB culture conditions.

Figure 3

A) Effect of treatment with 1,25D (10 nM, 6 hrs) on mRNA for osteocalcin, and the host (DAPK3 and LRP1) and target (COL4A1 and BMP2K) genes for miR-637 and miR-1228 respectively in passage 3 (P3) HOBS. Cells were analyzed prior to osteogenic stimulation (pre-stimulation) and in the presence (stimulated) and absence (unstimulated) of osteogenic treatment. B) Effect of treatment with 1,25D (0 -100 nM, 6 hrs) on mRNA for osteocalcin, and the host genes (DAPK3 and LRP1) and target genes (COL4A1 and BMP2K) for miR-637 and miR-1228 respectively in passage 11 (P11) HOBS. Data and error bars represent mean ± SEM of arbitrary PCR units (n=3; for 3A and 3B) for 3A * = p≤0.05; Student t test, for 3B and 3C * = p≤0.05; 2-factor ANOVA with Bonferroni post hoc test.)