Figure 4.

MTG16 contributes to epithelial restitution after injury. Mtg16^−/− (n=14) or WT (n=17) mice were treated with 3% dextran sodium sulphate (DSS) (w/v) or water ad lib for 4 days followed by recovery on water for 3 days prior to euthanising. (A) Body weights (mean body weight ± SD) are shown at days 0, 4 and 7 as per cent change from baseline (day 0 weight). (B) Per cent difference in colon length. (C) H&E stained representative sections of Mtg16^−/− or WT Swiss-rolled colons. Images at right represent boxed area from 40× images at left. (D) Quantification of colonic injury represented as an aggregate histological injury score. (E) Ki67 immunohistochemistry. (F) Representative activated caspase-3 immunohistochemistry (left) after 4 days of 3% DSS treatment (Bar denotes 10 μM). Quantification of activated caspase-3 staining (right) (n=5 per genotype) (*p<0.05, **p<0.01, ***p<0.001: DSS-treated compared with control (H₂0) mice, ¶p<0.05, ¶¶¶p<0.001: Mtg16^−/− vs WT DSS, §§p<0.01: Mtg16^−/− compared with WT control mice).