Figure 6.

Exacerbated innate and adaptive immune responses in Mtg16^−/− mice following dextran sodium sulphate (DSS) injury. Mtg16^−/− (n=8) or WT (n=8) mice treated with water or 3% DSS (w/v) according to our injury-repair model (4 days 3% DSS ad lib followed by 3 days of water recovery). (A) Flow cytometric analysis using haematopoietic lineage-specific antibodies from WT (n=8) or Mtg16^−/− (n=8) colons. An unpaired two-tailed t test indicated that there was a significant increase in Gr1⁺, F4/80⁺, CD11c⁺, MHCII expressing CD11c⁺, CD4⁺ and IFN-γ expressing CD4⁺ cells in Mtg16^−/− colons. (B) Representative cell plot histograms of MHCII expressing CD11c and IFN-γ expressing CD4 populations. (C) RNA expression levels measured by qRT-PCR on Mtg16^−/− colons presented as fold change from WT (dashed line). Error bars represent SD, **p<0.01, ***p<0.001. (D) Multi-analyte profiling using the Luminex platform revealed increased INF-γ, IL-17, TNFα, IL-4, and IL-13 and chemokines MIP-1β and KC in Mtg16^−/− colons. (E) Epithelial-specific KC mRNA expression levels (*p<0.05, **p<0.01, ***p<0.001; unidimensional ANOVA).