Figure 3.

OBs were cultured alone or in the presence of MKs for 5 days. Some cultures were treated with 1 or 10 μl of RGDS (daily), while others remained untreated. MKs were removed by washing and OBs were lysed and resolved by SDS-PAGE. Western blotting shows a significant increase in Pyk2 protein levels in OBs co-cultured with MKs as compared to OBs cultured alone. Treatment of OBs with RGDS did not alter Pyk2 levels compared to untreated OBs. Likewise, treatment of OBs co-cultured with MKs did not alter Pyk2 levels compared to untreated co-cultures. Representative blot from 2 experiments conducted.