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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Bone Miner Res. 2013 Jun;28(6):1434–1445. doi: 10.1002/jbmr.1876

Figure 4.

Figure 4

OBs were cultured in the presence or absence of MKs, and viable OB number (A) was determined by counting following trypan blue exclusion (n=6/experimental group) and Pyk2 expression (B) was determine by Western blot (n=3/experimental group). In some groups MKs were removed (by washing) after 4 hrs of co-culture (OB+MK washed). Priming OBs with MKs for 4 hrs is sufficient to significantly enhance OB number and Pyk2 expression 5 days later (OB+MK washed vs. OB washed or OB). Data reported are representative from 3 experiments conducted. *Indicates statistically significant difference (p<0.05) at the same time point compared to OB and OB washed. ΨIndicates statistically significant difference (p<0.05) at the same time point between OB+MK washed and OB+MK.