Figure 5.

OBs were cultured in the presence or absence of MKs for 5 days. In some groups MKs were removed (by washing) after 4 hrs of co-culture (OB+MK washed). Western blotting demonstrates that p53 expression was significantly reduced in OBs co-cultured with MKs for 5 days (OB vs. OB+MK). Of interest, priming OBs with MKs for 4 hrs (OB washed vs. OB+MK washed) is also sufficient to significantly reduce p53 protein expression. Representative blot from 3 experiments conducted.