Table 1.

Quantification of cytokines and mRNA levels in Mesothelioma implanted tumors

AB12 tumors
	Fold change in mRNA expression		Cytokine level (pg/mL)
	FP-ctrl	FP-surv	FP-ctrl	FP-surv
IL-6	0.023 ± 0.006	0.041 ± 0.012	5.267 ± 0.606	10.76 ± 1.722
IFN-γ	5.780 ± 1.624	20.889 ± 8.201*	1.516 ± 0.156	3.454 ± 0.455*
MCP-1	9.854 ± 1.275	17.398 ± 4.635	671.37 ± 130.87	1191.01 ± 293.40
TNF-α	0.830 ± 0.591	0.924 ± 0.264	1.618 ± 0.118	1.902 ± 0.156
IL-10	3.271 ± 0.830	5.219 ± 2.267	5.701 ± 0.059	12.054 ± 0.051*
IL-12	0.124 ± 0.018	0.713 ± 0.137*	11.334 ± 0.449	23.004 ± 1.313*

CRH5 tumors
	Fold change in mRNA expression		Cytokine level (pg/mL)
	FP-ctrl	FP-surv	FP-ctrl	FP-surv
IL-6	0.005 ± 0.001	0.025 ± 0.017	0.652 ± 0.050	2.851 ± 0.758
IFN-γ	0.145 ± 0.051	1.025 ± 0.236*	0.501 ± 0.256	3.654 ± 0.757*
MCP-1	2.664 ± 0.206	2.474 ± 0.228	113.12 ± 87.176	459.64 ± 9.385*
TNF-α	0.192 ± 0.071	0.097 ± 0.010	1.267 ± 0.202	1.806 ± 0.157*
IL-10	0.031 ± 0.008	0.951 ± 0.603	5.304 ± 0.081	8.023 ± 0.118*
IL-12	0.489 ± 0.186	0.053 ± 0.011	9.756 ± 2.374	9.634 ± 3.158

NOTE: Mesothelioma tumors (n = 5 for each treatment group) from control and survivin-vaccinated mice were harvested, homogenized and cytokine levels were quantified. DNA-free RNA was extracted from each tumor sample, reverse transcribed and subjected to real-time RT-PCR analysis. RNA was normalized using a stable housekeeping RNA (HPRT).

^*P < 0.05