Table 3.

Results of genetic association testing using 30 gene-wide SNPs in CRHR1

(A) Multiple regression analysis of overall genetic models with exon 6 SNPs fixed
	Phenotype
	Anxioustemperament	Hippocampus
	P =	P =
Additive	NS	0.001
D-major	0.014	0.008
D-minor	0.034	0.028

(B) Multiple regression analysis testing 30 SNPs simultaneously with exon6 SNPs fixed in model
	Phenotype
	Anxioustemperament		Hippocampus
	P=	Geneticmodel	P=	Geneticmodel
SNP5886	0.043	D-minor	NS
SNP9009	NS		0.044	D-minor
SNP2107	0.017	D-major	0.017	D-major
SNP0879	0.012	D-minor	0.021	Add., D-minor
SNP4820	NS		0.048	D-minor
SNP5137	NS		0.015	D-minor

Abbreviations: CRHR1, corticotrophin-releasing hormone receptor 1; SNP, single nucleotide polymorphism.

After taking linkage disequilibrium into account, 30 SNPs were assessed for association with anxious temperament (AT) and hippocampal metabolism using a multiple regression approach. The SNPs that showed significant association in the previous seven SNP analysis were held fixed in the model (SNP4805, SNP5043 and SNP5094). (A) The significance of the full model for each of the three alternative models (additive (Add.), dominance-major (Dmajor) and dominance-minor (D-minor)) was assessed for AT and hippocampal metabolism. (B) For each multiple regression model that was significant, the partial regressions (SNPs) were assessed. When multiple models were significant for the same SNP, the lowest P-value is presented.

Bolded P-values represent P≤0.05.