Fig 2.
TEM time course of aggreation in ammonium acetate, pH 7.0 containing heparin (25 μM). (a) At time zero, R2/wt has a combination of round aggregates and short fibers. (b) R2/wt fibers are elongated after 1h, but many exhibit a beaded appearance. (c) Numerous well-formed R2/wt fibers are present after 24h. For R2/ΔK280, the time zero sample (d) showed many short fibers and some round aggregates. (e) Many R2/ΔK280 fibers were present after 1h, and these were not beaded. (f) Numerous R2/ΔK280 fibers were present after 24h. Scale bars = 500 nm
