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a-c Mouse embryonic stem cell line ASE-9005 cultured on Transwell membrane inserts, with a human endometrial cell feeder layer in the outer well. Colonies from passage 44 shown 24, 48 and 72 h after seeding displayed typical ESC colony morphology. Colonies measuring 80–100 μM were selected for trypsinization and further expansion. d Typical morphology of mESC cultured on a MEF feeder layer is shown 72 h after plating
