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. 1973 Jun;51(6):1033–1036. doi: 10.1104/pp.51.6.1033

Ethylene-induced Phenylalanine Ammonia-Lyase Activity in Carrot Roots 1,2

Edo Chalutz a
PMCID: PMC366398  PMID: 16658459

Abstract

Ethylene enhanced the activity of phenylalanine ammonialyase in carrot (Daucus carota L., var. “Nauty”) root tissue. Slight increase in enzyme activity was exhibited by root discs incubated in ethylene-free air. It was probably due to the ethylene formed within the sliced tissue. Addition of ethylene to the air stream increased phenylalanine ammonia-lyase activity and the total protein content of the discs until maximum activity was reached after 36 to 48 hours of incubation. The continuous presence of ethylene was required to maintain high level of activity. Ethylene, at a concentration of 10 microliter per liter induced higher activity than at lower or higher concentrations. CO2 partially inhibited the ethylene-induced activity. Cycloheximide or actinomycin D effectively inhibited the ethylene-induced activity in discs that had not previously been exposed to ethylene. The results appear to support the hypothesis that the mode of action of ethylene may involve both de novo synthesis of the enzyme protein and protection or regulation of activity of the induced enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeles F. B., Holm R. E. Enhancement of RNA synthesis, protein synthesis, and abscission by ethylene. Plant Physiol. 1966 Oct;41(8):1337–1342. doi: 10.1104/pp.41.8.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chalutz E., Devay J. E., Maxie E. C. Ethylene-induced Isocoumarin Formation in Carrot Root Tissue. Plant Physiol. 1969 Feb;44(2):235–241. doi: 10.1104/pp.44.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engelsma G., van Bruggen J. M. Ethylene production and enzyme induction in excised plant tissues. Plant Physiol. 1971 Jul;48(1):94–96. doi: 10.1104/pp.48.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hyodo H., Yang S. F. Ethylene-enhanced Synthesis of Phenylalanine Ammonia-Lyase in Pea Seedlings. Plant Physiol. 1971 Jun;47(6):765–770. doi: 10.1104/pp.47.6.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Imaseki H. Induction of peroxidase activity by ethylene in sweet potato. Plant Physiol. 1970 Jul;46(1):172–174. doi: 10.1104/pp.46.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KOUKOL J., CONN E. E. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem. 1961 Oct;236:2692–2698. [PubMed] [Google Scholar]
  7. Riov J., Monselise S. P., Kahan R. S. Ethylene-controlled Induction of Phenylalanine Ammonia-lyase in Citrus Fruit Peel. Plant Physiol. 1969 May;44(5):631–635. doi: 10.1104/pp.44.5.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shannon L. M., Uritani I., Imaseki H. De novo synthesis of peroxidase isozymes in sweet potato slices. Plant Physiol. 1971 Apr;47(4):493–498. doi: 10.1104/pp.47.4.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stahmann M. A., Clare B. G., Woodbury W. Increased disease resistance and enzyme activity induced by ethylene and ethylene production of black rot infected sweet potato tissue. Plant Physiol. 1966 Nov;41(9):1505–1512. doi: 10.1104/pp.41.9.1505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zucker M. Sequential Induction of Phenylalanine Ammonia-lyase and a Lyase-inactivating System in Potato Tuber Disks. Plant Physiol. 1968 Mar;43(3):365–374. doi: 10.1104/pp.43.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]

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