Figure 1.
Maritime pine (Pinus pinaster) needles and grapevine (A, D, G), ethanol-acetone (B, E, H) and TCA-acetone (C, F, I) extraction methods from cork oak ECM roots, Martime Pine (Pinus pinaster) needles and Grapevine (Vitis viniferacv Regent) leaves. Proteins were separated on a 4–7 linear pH gradient in the first dimension (IEF) and 15% polyacrylamide gels in the second dimension.