Table 1.
Protein yields and total number of 2-DE protein spots, from grape leaves, pine needles, and cork oak ectomycorrhizal ECM roots after phenol, ethanol and TCA-acetone extraction protocols
Plant species | Protocol | Protein yield (mg/g FW)a | Total number of spots |
---|---|---|---|
Pine | Phenol | 5.81 ± 0.46 | 805 |
Ethanol | 21.88 ± 4.00 | 392 | |
TCA-acetone | 13.86 ± 1.14 | 657 | |
Grapevine | Phenol | 3.78 ± 0.61 | 532 |
Ethanol | 20.55 ± 1.79 | 166 | |
TCA-acetone | 16.57 ± 1.31 | 362 | |
Oak | Phenol | 0.61 ± 0.14 | 904 |
Ethanol | 3.57 ± 0.20 | 111 | |
TCA-acetone | 2.77 ± 0.14 | 36 |
a Mean and standard deviation from three technical replicates.
FW, fresh weight.