Fig. 2.
Maintenance of T. cruzi drug resistance after its cultivation without drug pressure. T. cruzi epimastigotes from the parental line and the resistant lines M15 (a) or B15 (b) were cultivated in vitro for 6 months without drug pressure. The concentrations of 2-Meotio or benznidazole that kill 50 % of the parasites (EC50) were calculated monthly to determine whether the resistance was maintained. Control refers to the resistant parasites obtained after the 15th passage in the presence of each compound. Asterisk, significant differences between the EC50 values for the M15 and B15 lines compared to the parental line