Fig. 3. Immunogold electron microscopy of monocarboxylate transporter 2 (MCT2) on perivascular astrocyte endfeet membranes (green) and on astrocyte plasma membranes in the neuropil (yellow).
Dense labeling of membrane associated MCT2 (red arrowheads) is present along the perivascular astrocyte endfoot facing the basal lamina (stippled line) in non-MTLE (A), whereas labeling of the same compartment is significantly reduced in MTLE (C). MCT2 labeling is also found on astrocytes plasma membranes in the neuropil, and to a lesser extent on organelles in the cytoplasm of these cells (B, D). Compared to non-MTLE (B), the density of MCT2 on plasma membranes of astrocytes in the neuropil is significantly upregulated in MTLE (stippled line in D). Abbreviations: astro, astrocytes; AT, axon terminal; bl, basal lamina; end, endothelial cell; lu, lumen; m, mitochondria. Arrow, bundle of intermediate filaments. Bars: 500 nm.