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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2010 Feb;46(2):87–91. doi: 10.1007/s11626-009-9248-8

Figure 1.

Figure 1

Characterization of ONSC cells as OPN-null SCCs. (A) ONSC cells lack the Spp1 gene. PCR analyses were performed with DNA purified from ONSC cells and from OPN heterozygous (+/-) mice. Lane M, DNA molecular marker; 600 bp indicates OPN+ alleles; 500 bp indicates OPN- alleles; Spp1, OPN gene. (B) Northern blot analysis of OPN mRNA. Total RNA extracted from JB6 Cl41.5a, CC4B, and ONSC cells was probed with radiolabeled OPN or β-actin cDNA, used as a loading control (lower panel). The observed OPN in the lower panel is due to non-stripped radiolabeled OPN probe. (C) Comparison of cell morphology with epidermal cell lines. ONSC, an OPN-null cutaneous SCC line; CC4A, an undifferentiated spindle cell carcinoma cell line; CC4B, a well-differentiated cutaneous SCC cell line; JB6 Cl41.5a, a preneoplastic mouse epidermal-like cell line. Magnification, ×200. (D) Immunohistochemical analyses for the expression of p63, K1 and K14 in ONSC cells. ONSC cells were immunostained with anti-p63, K1, and K14 antibodies or their isotype controls (IgG). Magnification, ×200; ×400 (inserted panel).