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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Mar 19;269(1):61–71. doi: 10.1016/j.taap.2013.03.006

Figure 4. Chromium induces BEAS-2B cell invasion.

Figure 4

For invasion assay, cells were treated with or without Cr(VI) at 0.5 μM for 6 weeks. 1 × 105 cells from each group were seeded on the top chamber of 24-well plate culture inserts coated with 20 μl of matrigel in duplicate. Cells were cultured for additional 72 hours, invaded cells on the bottom of insert were stained, photographed, and counted (A, B). Quantitative results were compared with control cells without Cr(VI) treatment, *P<0.01 when compared with control (B). For MMP-9 expression assay (C), cytosolic proteins were extracted from cells that were treated as mentioned above, 20 μg of proteins were separated on 10% SDS-polyacrylamide gel to detect MMP-9 expression, anti-β-actin antibody was probed to monitor protein loading. Blots are representative of three separate experiments with similar results, arrows indicate specific band.