Figure 2.

Acute effects of collagenase addition. (A) Representative images of mESCs after four days of growth in the indicated conditions. Scale bar = 200μm. (B) mRNA expression levels of self-renewal and differentiation markers after four days in the indicated conditions in the presence of serum. (C) mRNA expression levels of self-renewal and differentiation markers after four days in the indicated conditions in serum-free media. (D) mRNA expression levels of Fgf5 and Nanog, x-axis numbers indicate the MMP1 concentration added in ng/ml. (E) Heatmap comparing RNA-seq data for genes in the gene ontology list of stem cell maintenance-related genes from cultures of cells grown with LIF/BMP4 (LB), MMP1, neither, or both, clustered hierarchically using a centroid linkage after median normalization and log₂ transformation. (F) Fgf5 mRNA expression from cells grown in the presence of other recombinant MMPs. (G) mRNA expression levels of self-renewal markers for cells grown for two passages in the presence or absence of Ro32-3555. **=p<0.001, * =p<0.05; #=p<0.05 for all pairwise comparisons, all data represents averages of at least three independent experiments and error bars represent SD.