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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Stem Cells. 2013 Jun;31(6):1097–1106. doi: 10.1002/stem.1360

Figure 6.

Figure 6

Endogenous gp130-JAK-Stat3 signal. (A) Representative images of ESCs growing for the indicated number of passages in the presence of a LIF blocking antibody with added LIF or added MMP1. Scale bar = 200μm. (B) mRNA expression levels of indicated markers after four passages in the presence of MMP1 with or without LIF-blocking antibody. (C) RPKM values from normalized RNA-sequencing data for gp130 ligands in self-renewing mESCs. (D) mRNA expression levels of a Stat3 target gene after 1 hour of indicated additions. O/C/C indicates blocking the three ligands OSM, CT1, and CNTF. (E) CNTF levels recovered from conditioned media and solubilized ECM samples from cultures grown in the indicated conditions. (F) mRNA expression levels of self-renewal and differentiation markers in cells grown for four days in serum-free media with the indicated additions. (G) CNTF mRNA expression levels in the indicated cell types. (H) Model depicting the effect of matrix remodeling on mESCs. Dotted line indicates action of an unknown pathway. **=p<0.001, * =p<0.05; #=p<0.05 for all pairwise comparisons, all data represents averages of at least three independent experiments and error bars represent SD.