FIGURE 1. Our orthotopic implantation model allows for quantification of overall tumor burden and survival analysis.
(A) Representative bioluminescent images after orthotopic implantation of 1×105 4T1-luc2 cells. Bioluminescence images at 1, 8, 12 and 29 days after implantation demonstrate progressive tumor growth and metastatic spread. (B) The total tumor burden is quantified as total photons measured by bioluminescent technology as cancer progressed after implantation. (C) Kaplan-Meier survival curve of this model.