Figure 2. Decreased hematopoiesis in Pim1^−/− mice, but not in Pim2^−/− or Pim3^−/− mice.

(A) Lower frequency of BM CFU-GEMMs in Pim1^−/− mice, but not in Pim2^−/− or Pim3^−/− mice. BM cells were plated in MethocultR GF 3434 and CFU-GEMMs were determined at 12–14 days (*p<0.05, data are representative of 3 independent experiments). (B) Reduced animal survival in primary non-competitive BM transplant mice receiving Pim1^−/− BM cells. Lethally irradiated female FVB/J mice were injected via tail vein with BM cells obtained from male WT, Pim1^−/−, Pim2^−/−, or Pim3^−/− mice (5×10⁵ cells/recipient mouse). Animal survival was monitored daily (n=7/group, *p<0.05). (C) Reduced donor cell engraftment in primary non-competitive transplant mice receiving Pim1^−/− BM cells. Peripheral blood samples from animals in Fig. 2B were analyzed for donor cell engraftment as described (n= 4 for Pim1^−/− mice and n= 6 for other genotypes, *p<0.05). (D) Reduced animal survival in secondary BM transplant mice receiving Pim1^−/− BM cells. The primary transplant recipient mice as described in Fig. 2C were sacrificed at 4 months post transplant and BM cells harvested and injected into lethally irradiated female FVB/J mice (1×10⁷/recipient mouse). Animal survival was monitored daily (n=6/group). (E) Reduced donor cell engraftment in secondary transplant mice receiving Pim1^−/− BM cells. Secondary transplant recipient mice were sacrificed at 4 months post transplant and male donor cell engraftment in BM was analyzed (n=6/group, n=3 for Pim1^−/− in Fig. 2E, *p<0.05, **p<0.01). (F) Reduced donor engraftment in competitive BM transplant mice receiving Pim1^−/− BM cells. Competitive BM transplantation was performed as described using WT, Pim1^−/−, Pim2^−/−, or Pim3^−/− BM cells. Male donor engraftment in peripheral blood at 12 weeks post transplant was analyzed (n=7, **p<0.01).