Table 1.

Effect of different compounds on the distribution of GFP-wtVAP-1 in different cell types

Compound	Action	LX-2	aLMF	HSEC
Semicarbazide	SSAO inhibitor	X	X	X
Methylamine	SSAO substrate	X	X	X
Brefeldin A	Golgi/endosome inhibitor	Majority of GFP signal retained in Golgi
Monensin	Golgi inhibitor	X	X	X
Jasplakinolide	Actin inhibitor	X	Minor changes in distribution of GFP signal
Cytochalasin D	Actin inhibitor	GFP and VAP-1 distribution largely unaffected relative to cell shape. Association with cell protrusions more pronounced
Nocodzaole	Microtubule inhibitor	X	Minor changes in distribution of GFP signal
Marimastat	Metalloproteinase inhibitor	X	X	X
Bafilomycin A1	H + -ATPase inhibitor	Induced co-localization of GFP and anti-VAP-1 signal

“X” denotes no gross effect on distribution of GFP signal