Skip to main content
. Author manuscript; available in PMC: 2013 May 26.
Published in final edited form as: Arch Biochem Biophys. 2005 Jul 15;439(2):165–174. doi: 10.1016/j.abb.2005.04.022

Fig. 3.

Fig. 3

Irreversible loss of BMR activity following incubation under reaction conditions. The reaction conditions for all time courses were 5.0 nM BMR, 20 μM cyt c, and 50 μM NADPH at 30 °C. All reactions were initiated upon addition of NADPH. (a) The activity of BMR toward cyt c was determined from a 500 nM BMR solution stored at 4 °C. (b) After 1 h incubation under reaction conditions, BMR activity was lost. (c) BMR incubated under reaction conditions for 1 h was reconcentrated to 500 nM BMR like the solution used previously. The activity for this sample is shown.