Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences used for nested real-time PCR
| Nested PCR assay | Sequence | PCR product size (bp) | ||
|---|---|---|---|---|
| First-step PCR (external primer) |
Exon 14 |
forward |
TCTTGGAATGGCCTTGGAAGC |
282 |
| Exon 17 |
reverse |
TGAAACAGCATCACTTATGATGC |
||
| Second-step PCR (internal primer) | Exon 15 |
forward |
TGAAGAACCTCAAGGAGTGAAG |
154 |
| Exon 16 | reverse | TTTCTGTATTTTCCTCAGCGCT | ||
