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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2013 Mar 25;190(9):4508–4515. doi: 10.4049/jimmunol.1202923

FIGURE 7.

FIGURE 7

Peptides carrying PDK1 Thr-513 influence TCR-CD28-mediated mouse primary CD4+ T-cell activation. A, Uptake of PTD-conjugated peptides was analyzed by flow cytometry with FITC-conjugated peptides. B, Localization of PTD-conjugated peptides was analyzed by fluorescence microscopy with FITC-conjugated peptides. C and D, CD4+ T cells were isolated from C57BL6 mice and activated with anti-CD3 and anti-CD28 antibodies for the indicated times. Cells were stained with fluorochrome-conjugated anti-CD4, anti-CD69, and anti-CD25 antibodies. Surface expression of CD69 (C) and CD25 (D) on CD4+ T cells was analyzed by flow cytometry.