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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Dig Dis Sci. 2013 Jan 31;58(6):1516–1527. doi: 10.1007/s10620-013-2557-5

Figure 2. Expression of neuronal markers and channels in IM-FEN cultured at 39°C (3 days) compared to cells cultured at 33°C.

Figure 2

(A) RNA was isolated from IM-FEN cultured at 33°C or 39°C (3 days) and amplified for cRet, Kcnn4, NaV_1.3, TASK1, Clca1, and GAPDH (loading control), n=3. The two lanes/gene correspond to RNA isolated from cells grown at 33°C and 39°C. (B) Histogram for IM-FEN mRNA assessed by Real Time PCR showing the relative level of expression of various channels and neuronal markers, Peripherin and Ret after normalization to GAPDH expression. Results are mean ± S.E., n=3. **P < 0.01, ***P < 0.001.