Fig. 2.
Valproic acid inhibits CD8+ T-lymphocyte noncytolytic suppression at different levels of HIV-1 gene expression. CD4+ T-lymphocytes were infected with four different amounts of HIV-1NL4-3 pseudotyped virus to generate a titration of HIV-1 replication from 1.6×106 to 0.08×106 relative light units. JR-HVS CD8+ T-lymphocytes were cultured in direct contact at a 2:1 effector to target ratio with CD4+ T-lymphocytes infected with each of the different amounts of HIV-1NL4-3 pseudotyped virus. Valproic acid was added to the growth media in increasing concentrations. HIV-1 replication in the CD8+ T-lymphocyte co-culture is shown as the percentage of replication in the infected CD4+ T cells cultured alone. Mean and standard error of duplicate experiments are shown.