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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Immunol. 2013 Apr 21;14(6):593–602. doi: 10.1038/ni.2576

Figure 2.

Figure 2

miR-155 is required for CD8+ T cell responses. Reduced CD8+ T cell responses (a), (b) and viral clearance (c) in influenza virus-infected miR-155-KO mice. (a) Representative flow cytometric plots and (b) numbers of day 10 lung NP(366–374)-specific CD8+ T cells shown. (c) Day 10 lung viral loads of wild-type and miR-155-KO mice. Data are from three experiments. * P < 0.002 and ** P < 0.05 (Student’s t-test). (d) , (e) , (f) Impaired response of miR-155-KO OT-I cells to WSN-OVA influenza virus. Representative flow cytometric plots of lung (d), and numbers (e) of donor OT-I cells in day 10 lungs, mediastinal LN (MLN) and spleens shown. Data from 4 experiments. * P < 0.001 (Student’s t-test for lungs and spleens, Mann-Whitney U test for MLN). (f) Kinetics of lung donor OT-I cell numbers shown (two experiments, n = 4 per group). (g) and (h) Reduced response of miR-155-KO OT-I against Listeria monocytogenes infection. Representative flow cytometric plots of spleens (g) and numbers (h) of day 7 post-infection donor IFN-γ+ OT-I cells in spleens and mesenteric LN are depicted. Data from two experiments. * P < 0.001 (Student’s t-test) (i) and (j) miR-155 deficiency impairs CD8+ T cell memory generation. Representative flow cytometric plots of spleens (i) and numbers in spleens (j) of day 60 donor memory OT-I cells in WSN-OVA influenza virus-infected mice shown. Means and SEM of 2 experiments ( n = 6 per group) shown. * P < 0.05 (Mann-Whitney U test). In all figures above, numbers in flow cytometric plots indicate percent of lymphocytes.