Figure 3.

Effect of deoxyshikonin on VEGF-C, -A, and -D mRNA levels and regulation of HIF-1α during cord formation of HMVEC-dLy on Matrigel. Cells were exposed with or without 0.8 μM deoxyshikonin for 6 h and then underwent experiments. (a) The relative length of cords was measured using an Angiogenesis Image Analyzer. (b), (c) VEGF-C, -A, and -D and also HIF-1α mRNA levels were detected by real-time PCR. (d) The HIF-1α protein level was determined by immunoprecipitation and Western blotting. The results were analyzed by scanning and Scion Image software. (e) HIF-1α nuclear translocation as determined by immunofluorescence microscopy. Similar results were obtained in three independent experiments; *P < 0.05, **P < 0.01 compared with their control.