Figure 6.

Decreased GATA3 Expression Is Essential for Early B Cell Development

(A) Sorted e14.5 WT fetal liver LSKs were transduced with only pMX-6ZFP or MigY-EBF1 or both viruses, plated on OP9s, and analyzed for frequencies of viable B220⁺CD19⁺ cells on day 7. Double-transduced cells were identified as viable (DAPI⁻) GFP⁺YFP⁺ cells.

(B) Sorted CD45^B6+Flt3⁺ LSKs and CLPs from B6.CD45^SJL adults previously reconstituted with BM cells from C57BL/6 (CD45^B6) backcrossed Vav^Cre or Vav^CreGata3^fl/fl mice were transduced with pMX or pMX-6ZFP. Equal numbers of GFP⁺ cells were sorted into triplicate cytokine supplemented OP9 stromal cultures, and B cell differentiation was assessed 7 days later. Graphical data are means ± SEMs of triplicate samples.

p < 0.001.