Fig. 1.

Expression of mRNA of Nrf2 and Nrf2 target genes in choroid plexus after systemic LPS injection. The mRNA expression of Nrf2 and its gene targets were analyzed in choroid plexuses of PND9 mice after i.p. injection of low dose LPS (0.3 mg/kg) at 2–72 h (n=8–16 per group) and at 24 h after high dose LPS (1 mg/kg; n=8). Reverse transcription-quantitative PCR analysis showed a down-regulation of Nrf2 expression at 24 h after injection with both LPS doses (a). Similarly, at 24 h both LPS doses reduced the mRNA of Gclc (b). Gclm was upregulated by low dose of LPS at 2 h while it was down-regulated by high LPS dose at 24 h after treatment (c). A dual phase effect was seen for mRNA of HO-1 after low dose of LPS: mRNA was strongly increased both at early survival times (2 and 6 h) and late time point (72 h). No effect was on HO-1 seen with the higher LPS dose 24 h following the LPS injection (d). Data are shown as fold change over control±SEM, ^*p≤0.05, ^**p≤0.01, ^***p≤0.001