Table 1.
Immunohistochemistry: information on secondary antibodies used
| Antibody | Type | Clone/number | Titration | Source |
|---|---|---|---|---|
| FDC-SP | Rabbit polyclonal | Anti-C4 or f7 HPA014326 | 1:80 | Sigma, St Louis, MO, USA |
| ODAM | Rabbit polyclonal | HPA036543 | 1:10 | Sigma, St Louis, MO, USA |
| CK 8 | mouse monoclonal | Supernatant | 1:5 | CRUK |
| CK 10 | IgG-1 | – | 1:50 | DAKO A/S, Glostrup, Denmark |
| CK 13 | IgG-1 | KS-1A3 | 1:50 | Novocastra |
| CK 16 | Mouse monoclonal | Supernatant | 1:5 | CRUK |
| CK 19 | Mouse monoclonal | Supernatant | 1:5 | CRUK |
| Ki-67 | IgG-1 | MIB-1 | 1:25 | DAKO A/S, Glostrup, Denmark |
| Filaggrin | IgG-1 | 15CID | 1:50 | Monosan |
| TG | Rabbit polyclonal | pab0061 | 1:250 | Covalab-UK |
| Collagen IV | IgG-1 | CIV 22 | 1:50 | DAKO A/S, Glostrup, Denmark |
| Laminin-1 | IgG-2a | 4C7 | 1:1200 | DAKO A/S, Glostrup, Denmark |
CRUK, Cancer Research United Kingdom (Abs were a kind gift from Prof. I. C. Mackenzie, Institute for Cell and Molecular Science, London, UK).