Fig. 2.

Growth differences between delta-like 1 homologue (Dlk1)-null mutant and wild-type littermate mice and analyses of Dlk1-null mutant in late embryonic development. (a, i) Weights of male and female wild-type and Dlk1-null mutant mice from weaning at 3 weeks to 14 weeks of age. Males have a statistically significantly reduced weight at the majority of timepoints, and females have a lower weight from weaning to 14 weeks of age, although their rate of growth appears normal. (a, ii) Nose-to-tail measurements of the body length of wild-type and Dlk1-null mutant mice at 14 weeks of age. (b, i) Weights of E18.5 mutant embryos and their wild-type counterparts at E18.5. (b, ii) Pituitaries of Dlk1-null mutant mice and wild-type littermates were assayed for growth hormone (GH) content. (a) Data shown are the mean ± SEM of 7–10 mice. (b) Data shown are the mean ± SEM of 12–13 mice. Statistical analysis by mixed-effects model of weight versus age using genotype as fixed factors and subjects (mice) as random factors, with anova to test the overall effect of genotype on growth followed by Tukey's post-hoc tests (a) and unpaired t-test (b). *P < 0.05; **P < 0.01; ***P < 0.001 Dlk1-null versus wild-type littermates.