Figure 3.

Localization of vasa and dnd transcripts or Ly75 protein in the genital ridge of Atlantic salmon larva. In situ hybridization with vasa (anti-sense: A and C; sense: B and D) or dnd (anti-sense: E and G; sense: F and H) probes, and immunohistochemistry with Ly75 antibody (I and K) or without primary antibody (J). (A, B) and (C–K) are hatching (83 dpf) and yolk-sac resorption stages (139 dpf), respectively. (K) is a high-magnification view of genital ridge area enclosed by dashed box in (I). Embryos were fixated with PFA (A, B, E, F, and I–K) or Bouin's solution (C, D, G, and H). Arrowheads indicate the genital ridges. g, gut; m, mesonephric duct. [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com]