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. Author manuscript; available in PMC: 2013 May 27.
Published in final edited form as: Curr Drug Targets. 2008 Feb;9(2):123–129. doi: 10.2174/138945008783502430

Fig. (3. Syk and Src are required for optimal TNF-α production in M. smegmatis infected macrophages.

Fig. (3

Balb/C bone-marrow derived macrophages were left untreated or pre-treated with 10 μM “Syk inhibitor” or PP2 (Src inhibitor) (CalBiochem, LaJolla, CA) and/or with 50 μg/ml anti-Dectin-1 blocking antibody 2A11. 1 hour post-antibody or 20 minutes post-inhibitor treatment, the macrophages were infected with M. smegmatis. TNF-α released into the culture supernatant 24 hours post-infection was quantified by ELISA. Results represent mean ± SD of duplicate ELISA wells. The Syk and Src inhibitor experiments were performed independently. *P<0.05. UI: uninfected macrophages.