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. Author manuscript; available in PMC: 2013 May 27.
Published in final edited form as: Biochemistry. 2009 Apr 7;48(13):3005–3016. doi: 10.1021/bi802117k

Table 2.

Hill Equation Parameters for Fits to Calmodulin Concentration Dependence of Inhibition of Sarcoplasmic Reticulum Vesicle [3H]Ryanodine Binding

wild type L3 PEST L6 NP oxidized L6 NP wild type L5 NTerm oxidized L5 NTerm wild type L4 CTerm oxidized L4 CTerm
inhibition (% maximal binding) 12.1 ± 1.0 9.4 ± 1.2 9.2 ± 0.7 7.2 ± 1.4a 15.0 ± 0.8 10.7 ± 0.8a 7.2 ± 1.4a,b 17.5 ± 1.7 15.8 ± 1.1 10.3 ± 2.3a
nic −1.99 ± 0.53 −2.84 ± 1.33 −3.18 ± 0.93 −2.12 ± 1.41 −2.10 ± 0.38 −3.77 ± 1.34 −2.37 ± 2.09 −1.78 ± 0.53 −1.6 ± 0.34 −2.39 ± 1.8
IC50c(nM) 27.8 ± 4.0 35.8 ± 7.5 37.8 ± 4.7 20.5 ± 6.8 31.3 ± 3.0 30.9 ± 3.0 248.3 ± 96.1a,b 37.9 ± 7.4 53.7 ± 7.8 47.5 ± 18.8
nc 4 4 4 4 6 6 6 10 10 10
a

Significantly different (p < 0.05) from wild type.

b

Significantly different (p < 0.05) from unoxidized.

c

ni, the Hill coefficient of ryanodine binding; IC50, the concentration of CaM required to achieve half-inhibition of ryanodine binding; n, number of separate experiments performed.