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. 2013 May 27;8(5):e63777. doi: 10.1371/journal.pone.0063777

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© 2013 Deng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative plots showed Foxp3 expression in CD4⁺CD25^- T cells cultured with different media for 72 h. The supernatants of AGS cells cultured under normoxic or hypoxic condition were called normoxic medium and hypoxic medium, respectively, while AIM-V medium with or without recombinant human TGF-β1 (reTGF-β1) was named reTGF-β1 medium and control medium, respectively. (B) A histogram showed Foxp3 expression in CD4⁺CD25^- T cells cultured with different media. (C) A histogram showed that TGF-β1 receptor kinase inhibitor LY-364947 (TGF-β1 In) partly ablates the indution of Foxp3 in CD4⁺CD25^- T cells cultured in different media. Values represent mean ± SEM. * P<0.001.