Figure 6. Increased levels of cathepsin protease activity are reduced by bafilomycin A1 treatments.
A) Wildtype (AB) and vps11w66 zebrafish lysates were analyzed by PARP-1 immunoblot and densitometry analysis. vps11w66 lysates show higher levels of cathepsin cleavage products at 62 kDa in size as compared to wildtype fish. Treatment of larvae with Baf A1 reduces accumulation of the 62 kDa cleavage product. B) Graphical representation of density analysis of 62 kDa band from three immunoblots using Image J. Samples were analyzed via one way ANOVA with Bonferonni multiple comparison analysis (p<0.0001***). Error bars are standard deviation.