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. 2013 May 21;13:112. doi: 10.1186/1472-6882-13-112

Figure 3.

Figure 3

Inhibitory effect of WESS on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were pre-incubated with vehicle or WESS (25 μg/ml) for 3 h and further cultured in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. Total RNA and cell lysates were obtained at the indicated days. (A) c-Fos and NFATc1 mRNA levels were analyzed by QPCR. aP < 0.05, bP < 0.01, cP < 0.001 vs. vehicle-treated control group. (B) Total cell lysates were subjected to Western blot analysis with antibodies specific for c-Fos and NFATc1. GAPDH was used as a loading control. (C) BMMs infected with retroviruses expressing either pMX-IRES-GFP (pMX) or pMX-CA-NFATc1-IRES-GFP (pMX-CA-NFATc1) were cultured with vehicle or WESS (25 μg/ml) in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. The number of TRAP(+)MNC containing more than three nuclei with GFP expression were counted. Data represents mean ± SD of three independent experiments. cP < 0.001 vs. vehicle-treated control group.