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. 2013 May 21;13:112. doi: 10.1186/1472-6882-13-112

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Copyright © 2013 Ha et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Inhibitory effect of WESS on bone resorption activity of mature osteoclasts. Mature osteoclasts generated on collagen gels were replated on an Osteo Assay Surface plate and cultured with vehicle or WESS for 24 h. (A) Representative microscopic pictures of TRAP staining (upper panel) and resorption pits (down panel). (B) The number of TRAP(+)MNC containing three or more nuclei. (C) Relative resorption area. Data represents mean ± SD of three independent experiments. ^cP < 0.001 vs. vehicle-treated controls.