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. 2013 May 27;8(5):e64980. doi: 10.1371/journal.pone.0064980

Figure 7. Immunoblotting validates ssp1 as an empty container.

Figure 7

Duplicate one microgram aliquots of total seed protein from tester and backcross homozygotes were subjected to SDS-PAGE and immunoblotting with an anti-PHA antibody. Reporter lines 1, 3, 5, and 7 are shown in duplicate as testers (T) and the corresponding backcrosses (B). Aliquots of purified PHA were included as standards. The autoradiographs were scanned by densitometry and quantified by employing Image J software. The bar graphs represent the average PHA values relative to 30 ng of purified PHA. T-test statistical analyses revealed that lines 1, 3, and 5 have p-values less than 0.05, while the line 7 p-value was greater than 0.05. An independent experiment on the previous generation of these tester/backcross lines revealed similar enrichment trends and p-values.