Table 1. Resveratrol modulated proteins in MCF-7 breast cancer cells identified by ESI-MS/MS mass spectrometry.
| Protein namea (Spot number) | Molecular mass/pI | Gene nameb | Accession numberc | Mascot Score | Matched peptides | Sequence coverage | MS/MS peptide sequence (ion scores) | Molecular function/Cellular process |
| Translationally-controlled tumor protein (1102) | 19583/4.84 | TPT1 | P13693 | 507 | 7 | 71% | 39TEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTK85 (242) 131NYQFFIGENMNPDGMVALLDYR152 (103) 6DLISHDEMFSDIYK19 (69) 111VKPFMTGAAEQIK123 (81) 53EDGVTPYMIFFK164 (53) | Calcium ion binding/Calcium binding and microtubule stabilization |
| Triosephosphate isomerase (8101) | 26807/6.51 | TPI1 | P60174 | 533 | 9 | 78% | 18KQSLGELIGTLNAAK32 (105) 19QSLGELIGTLNAAK32 (92) 85DCGATWVVLGHSER98 (87) 69VTNGAFTGEISPGMIK84 (86) 206IIYGGSVTGATCK218 (82) | Triose-phosphate isomerase activity/Gluconeogenesis, Glycolysis, Pentose shunt |
| Stress-induced-phosphoprotein 1 (6604) | 68721/7.81 | STIP1 | G3XAD8 | 445 | 6 | 26% | 399LAYINPDLALEEK411 (69) 125KAAALEFLNR134 (62) 126AAALEFLNR134 (57) 581LMDVGLIAIR590 (52 561DPQALSEHLK570 (47) 454LLEFQLALK462 (43) | Stress-induced-phosphoprotein/Cellular network of molecular chaperones and folding catalysts. |
| Pyridoxine-5′-phosphate oxidase 1 (7303) | 30311/6.62 | PNPO | Q9NVS9 | 297 | 3 | 26% | 46EAFEETHLTSLDPVK60 (130) 164SSQIGAVVSHQSSVIPDR181 (101) 235GLPTGDSPLGPMTHR249 (74) 237PTGDSPLGPMTHR249 (54) 46EAFEETHLTSLDPVK60 (24) | Catalyzes oxidation/Pyridoxine biosynthesis |
| Hypoxanthine-guanine phosphoribosyl-transferase (7203) | 24590/6.24 | HGPRT1 | P00492 | 138 | 4 | 21% | 115VIGGDDLSTLTGK127 (96) 128NVLIVEDIIDTGK140 (56) 141TMQTLLSLVR150 (42)34VFIPHGLIMDR44 (16) | Guanine phosphoribosyltransferase activity; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding/Generation of purine nucleotides |
| Peroxiredoxin-6 (8204) | 25133/6 | PRDX6 | P30041 | 128 | 9 | 37% | 109ELAILLGMLDPAEK122 (63) 145LSILYPATTGR155 (58) 98LPFPIIDDR106 (49) 109ELAILLGMLDPAEKDEK125 (44) 85DINAYNCEEPTEK97 (42) 133VVFVFGPDK141 (41) 156NFDEILR162 (38) 133VVFVFGPDKK142 (36) 183DGDSVMVLPTIPEEEAKK200 (4) | Antioxidant; glutathione peroxidase; peroxiredoxin; phospholipase A2/Redox regulation; phospholipid turnover. |
| Heat shock protein beta-1 (6203) | 22427/7.83 | HSP27 | P04792 | 257 | 10 | 60% | 172LATQSNEITIPVTFESR188 (101) 97VSLDVNHFAPDELTVK112 (74) 141KYTLPPGVDPTQVSSSLSPEGTLTVEAPMPK171 (70) 80QLSSGVSEIR89 (64) 28LFDQAFGLPR37 (50) | Protein kinase C inhibitor activity/Stress resistance and actin organization |
a,b,c
Protein Knowledgebase (UniProtKB).